Reconstitution Guide – TAD — Timeless Age Defying Peptides

A laboratory reference for preparing lyophilised (freeze-dried) research peptides with bacteriostatic water, calculating the concentration of a prepared solution, converting between insulin-syringe units and millilitres, and storing material correctly.

Research use only. All TAD products are supplied strictly for in-vitro laboratory research and analytical use. They are not medicines and are not for human or veterinary use, consumption, or administration. Nothing on this page is medical, therapeutic, or dosing advice, and nothing here recommends or describes administration to a person or animal. The figures below are arithmetic worked examples of solution concentration only. It is the researcher’s responsibility to work within all applicable laws and institutional and safety requirements.

What reconstitution means

Peptides are supplied as a lyophilised powder in a sealed vial. Reconstitution is simply dissolving that powder into a known volume of liquid — bacteriostatic water is the usual diluent — so the amount of peptide held in any given volume of solution is known and consistent. The only variables are the mass of peptide in the vial (stated on the label) and the volume of diluent added. Those two numbers fix the concentration.

Calculating concentration

Concentration is the mass of peptide divided by the volume of diluent added:

Concentration (mg/ml) = peptide mass (mg) ÷ diluent volume (ml)

For example, a 10 mg vial reconstituted with 2 ml of bacteriostatic water holds 10 ÷ 2 = 5 mg/ml. The same 10 mg vial reconstituted with 3 ml holds 10 ÷ 3 = 3.33 mg/ml. Adding more diluent does not change the total amount of peptide in the vial — it only spreads it through a larger volume, so each millilitre is more dilute.

To express the same figure in micrograms per millilitre, multiply by 1,000: 3.33 mg/ml is 3,333 mcg/ml.

Insulin-syringe units and millilitres

A standard U-100 insulin syringe is graduated in “units”, where 100 units equal 1 millilitre. Each unit is therefore 0.01 ml. This is purely a volume conversion on the syringe barrel — it says nothing about how much material that volume should contain for any purpose.

1 unit (U-100) = 0.01 ml · 50 units = 0.5 ml · 100 units = 1.0 ml

The amount of peptide drawn into a given number of units is the concentration multiplied by that volume. At 3,333 mcg/ml, one unit (0.01 ml) contains 3,333 × 0.01 = 33.3 mcg.

Volume per unit at common concentrations

The table converts unit markings on a U-100 syringe into volume and into the mass of peptide that volume holds, across a range of prepared concentrations. It is a calculator, not a recommendation of any quantity.

Syringe units	Volume (ml)	at 1 mg/ml	at 2 mg/ml	at 3.33 mg/ml	at 5 mg/ml
5 units	0.05	50 mcg	100 mcg	167 mcg	250 mcg
10 units	0.10	100 mcg	200 mcg	333 mcg	500 mcg
20 units	0.20	200 mcg	400 mcg	667 mcg	1,000 mcg
50 units	0.50	500 mcg	1,000 mcg	1,667 mcg	2,500 mcg

Worked examples by vial size

The TAD range is supplied in several strengths. Reconstituting with different diluent volumes gives the concentrations below — again, arithmetic only, to show how vial strength and diluent volume interact.

Vial strength	Diluent added	Concentration	Per unit (0.01 ml)
5 mg	2 ml	2.5 mg/ml	25 mcg
10 mg	2 ml	5 mg/ml	50 mcg
10 mg	3 ml	3.33 mg/ml	33.3 mcg
40 mg	3 ml	13.3 mg/ml	133 mcg
100 mg	3 ml	33.3 mg/ml	333 mcg
500 mg	3 ml	167 mg/ml	1,667 mcg

Reconstitution technique

Good aseptic technique keeps a prepared solution stable and uncontaminated for the life of the vial.

Work on a clean surface with clean hands. Allow the lyophilised vial and the bacteriostatic water to reach room temperature.
Wipe the rubber stopper of both the peptide vial and the bacteriostatic water vial with a fresh alcohol swab and let them dry.
Draw the chosen volume of bacteriostatic water into a syringe.
Introduce the water slowly, letting it run down the inside wall of the vial rather than firing it directly onto the powder. The peptide is delicate; a forceful stream can degrade it.
Do not shake. Swirl gently, or leave the vial to stand, until the powder has fully dissolved into a clear solution.
Label the vial with its contents, concentration and the date of reconstitution.

Storage & stability

Unopened lyophilised vials are stable for extended periods and are best kept in a freezer, away from light. Once reconstituted, a vial should be refrigerated at 2–8 °C. Bacteriostatic water contains 0.9% benzyl alcohol, which suppresses microbial growth and is what allows a reconstituted vial to be kept and re-accessed over a number of weeks rather than discarded after a single use; sterile or plain water does not offer this and is not suitable for multi-use storage.

Keep reconstituted material out of direct light and away from repeated temperature swings. Avoid freezing a reconstituted solution, as freeze–thaw cycles can damage the peptide. Inspect before use and discard any solution that has become cloudy, discoloured or shows particulates.

A note on bacteriostatic water. Every TAD peptide order includes bacteriostatic water at no charge, matched to the number of vials ordered. Additional bacteriostatic water can be purchased separately from the Lab Supplies category.

Quality & certificates of analysis

Every batch is supplied with a certificate of analysis confirming identity and purity by HPLC. Batch certificates can be looked up in the Quality & COA centre. For anything not covered here, contact customerenquiries@tadpeptides.co.uk.

This reference describes laboratory preparation and handling technique only. It does not constitute medical, clinical, therapeutic or dosing advice, and it does not recommend or describe administration of any product to a person or animal. TAD products are for laboratory research use only and are not for human or veterinary use.